Methionyl Soluble Ribonucleic Acid Transforrnylase
نویسندگان
چکیده
The MO-formyltetrahydrofolate:methionyl soluble ribonucleic acid transformylase has been purified over 1500-fold from Escherichia coli B. Although not homogeneous at this stage, the enzyme was of sufficient purity to determine some physical characteristics. The sedimentation constant was 2.02 which would indicate a molecular weight of approximately 25,000. In addition to the substrates of the reaction a partial dependency on magnesium ion was observed. The identity of the formyl donor was elucidated and found to be No-formyltetrahydrofolate.
منابع مشابه
The Enzymic Synthesis of Amino Acyl Derivatives of Ribonucleic Acid I. THE MECHANISM OF LEUCYL-, VALYL-, ISOLEUCYL-, AND METHIONYL RIBONUCLEIC ACID FORMATION”
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